Studi perbandingan DNA barcoding untuk Bambu
DOI:
https://doi.org/10.70158/buitenzorg.v1i2.9Abstrak
Subfamili Bambusoideae, kelompok penting dalam famili Poaceae, mengandung beragam genus dengan hubungan taksonomi yang kompleks. Penelitian ini bertujuan untuk menjelaskan hubungan filogenetik dan keragaman genetik dalam bambu, dengan fokus pada kegunaan DNA barcoding, yaitu ITS2, matK, dan rbcL, dalam identifikasi spesies bambu. Dengan menganalisis data sekuens dari penanda ini, pohon filogenetik dibangun menggunakan metode kemungkinan maksimum untuk menyimpulkan hubungan evolusi di antara spesies. Hasil penelitian menunjukkan bahwa ITS memberikan resolusi tertinggi untuk identifikasi tingkat spesies, membedakan spesies yang berkerabat dekat secara lebih efektif daripada matK dan rbcL. Sementara matK menunjukkan klasifikasi tingkat genus yang kuat, rbcL dibatasi oleh konservasinya yang tinggi, sehingga lebih cocok untuk pengelompokan taksonomi yang lebih luas. Temuan ini berkontribusi pada pemahaman yang lebih baik tentang taksonomi bambu dan menyoroti pentingnya pemilihan penanda berdasarkan resolusi taksonomi yang diperlukan. Penelitian ini juga menekankan penggunaan pelengkap penanda ini untuk memberikan pandangan yang komprehensif tentang filogenetik bambu.
Kata kunci: taksonomi bambu, DNA barcoding, ITS2, matK, rbcL
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Referensi
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