Comparison of DNA extraction methods for bornean ironwood plant (Eusideroxylon zwageri)

Authors

  • Salsabila Al Alya Ridzqya Department of Biology, Faculty of Biology, Jenderal Soedirman University
  • Aqwin Polosoro Research Center for Genetic Engineering, National Research and Innovation Agency
  • Wening Enggarini Research Center for Genetic Engineering, National Research and Innovation Agency
  • Kusumawaty Kusumanegara Research Center for Horticulture, National Research and Innovation Agency
  • Hendra Helmanto Research Center for Ecology and Ethnobiology, National Research and Innovation Agency
  • Mahat Magandhi Research Center for Applied Botany, National Research and Innovation Agency
  • Dani Satyawan Research Center for Genetic Engineering, National Research and Innovation Agency
  • Toto Hadiarto Research Center for Genetic Engineering, National Research and Innovation Agency
  • Alice Yuniaty Department of Biology, Faculty of Biology, Jenderal Soedirman University

DOI:

https://doi.org/10.70158/buitenzorg.v1i1.7

Abstract

DNA isolation is a routine procedure in molecular analysis. The method of plant genome DNA extraction has been widely available throughout global laboratories. Several labs made some modifications to obtain optimal results. This research was aimed to analyse two types of DNA extraction methods for ironwood plant (Eusideroxylon zwageri), one tropical rain forest woody plant known for its high strength and durability. The results indicate that isolation DNA kit produced high DNA purity with lower concentrations while CTAB methods generated lower DNA purity with higher concentrations. This may be used as consideration of DNA isolation for downstream molecular analyses.

 

Keywords: DNA extraction, ironwood, CTAB, genomic DNA

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Published

29-06-2024

How to Cite

Ridzqya, S. A. A. ., Polosoro, A., Enggarini, W., Kusumanegara, K., Helmanto, H., Magandhi, M., Satyawan, D., Hadiarto, T., & Yuniaty, A. . (2024). Comparison of DNA extraction methods for bornean ironwood plant (Eusideroxylon zwageri). Buitenzorg: Journal of Tropical Science, 1(1), 25–32. https://doi.org/10.70158/buitenzorg.v1i1.7

Issue

Vol. 1 No. 1 (2024): Buitenzorg: Journal of Tropical Science

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