Perbandingan Metode Ekstraksi DNA pada Tanaman Ulin (Eusideroxylon zwageri)

Penulis

  • Salsabila Al Alya Ridzqya Department of Biology, Faculty of Biology, Jenderal Soedirman University
  • Aqwin Polosoro Research Center for Genetic Engineering, National Research and Innovation Agency
  • Wening Enggarini Research Center for Genetic Engineering, National Research and Innovation Agency
  • Kusumawaty Kusumanegara Research Center for Horticulture, National Research and Innovation Agency
  • Hendra Helmanto Research Center for Ecology and Ethnobiology, National Research and Innovation Agency
  • Mahat Magandhi Research Center for Applied Botany, National Research and Innovation Agency
  • Dani Satyawan Research Center for Genetic Engineering, National Research and Innovation Agency
  • Toto Hadiarto Research Center for Genetic Engineering, National Research and Innovation Agency
  • Alice Yuniaty Department of Biology, Faculty of Biology, Jenderal Soedirman University

DOI:

https://doi.org/10.70158/buitenzorg.v1i1.7

Abstrak

Isolasi DNA adalah prosedur rutin dalam analisis molekuler. Metode ekstraksi DNA genom tanaman telah tersedia secara luas di seluruh laboratorium global. Beberapa laboratorium melakukan beberapa modifikasi untuk mendapatkan hasil yang optimal. Penelitian ini bertujuan untuk menganalisis dua jenis metode ekstraksi DNA untuk tanaman kayu ulin (Eusideroxylon zwageri), salah satu tanaman kayu hutan hujan tropis yang dikenal karena kekuatan dan daya tahannya yang tinggi. Hasil penelitian menunjukkan bahwa kit DNA isolasi menghasilkan kemurnian DNA yang tinggi dengan konsentrasi yang lebih rendah sedangkan metode CTAB menghasilkan kemurnian DNA yang lebih rendah dengan konsentrasi yang lebih tinggi. Ini dapat digunakan sebagai pertimbangan isolasi DNA untuk analisis molekuler hilir.

 

Kata kunci: ekstraksi DNA, kayu ulin, CTAB, DNA genomik

Unduhan

Data unduhan belum tersedia.

Referensi

Abdurachman (2012) Tanaman Ulin (Eusideroxylon zwageri T. & B) pada Umur 8,5 Tahun di Arboretum Balai Besar Penelitian Dipterokarpa Samarinda. Info Teknis Dipterokarpa, 5(1), 25–33.

Ahmad, M. A., Rasol, N. E., Ab Ghani, N., Jim Shamsudin, K., & Suleiman, M. (2023). Flavonoids from the Borneo Plant Species: Eusideroxylon zwageri Teijsm. & Binn. Journal of Science and Mathematics Letters, 11(1), 39–42. DOI: https://doi.org/10.37134/jsml.vol11.1.5.2023

Apriyono, A., Melanie, A., Erfinanto, E., Fadjriah, N., Mahbub, H., Dominique, M., & Ramdania, el H. (2019). CERITA 100 POHON (A. Hamidi, F. N. Tirtaningtyas, & Y. I. D. Cahyo, Eds.).

Ariati, S. R., & Widyatmoko, D. (2019). Bogor Botanic Gardens. Sibbaldia: The International Journal of Botanic Garden Horticulture, (17), 11–28. DOI: https://doi.org/10.24823/Sibbaldia.2019.265

Attitalla, I. H. (2011). Modified CTAB Method for High Quality Genomic DNA Extraction from Medicinal Plants. Pakistan J. of Biological Sciences, 14 (21): 998-999.

Doyle, J.J. and Doyle, J.L. (1987) A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin, 19, 11-15.

Goto, S., Miyahara, F., & Ide, Y. (2002). Identification of the male parents of half-sib progeny from Japanese black pine (Pinus hunbergia Parl.) clonal seed orchard using RAPD markers. Breeding Science, 52(2): 71- 77.

Huang, J., Ge, X. and Sun, M. (2000) Modified CTAB Protocol Using a Silica Matrix for Isolation of Plant Genomic DNA, BioTechniques, 28(3): 432-434. DOI: https://doi.org/10.2144/00283bm08

Khosravinia, H., H. N. N. Murthy, D. T. Prasad, & N. Pirany. (2007). Optimizing Factors Influencing DNA Extraction From Fresh Whole Avian Blood. African Journal of Biotechnology, 6(4): 481-486.

Kiss, T., Karácsony, Z., Gomba-Tóth, A. et al. (2024) A modified CTAB method for the extraction of high-quality RNA from mono-and dicotyledonous plants rich in secondary metabolites. Plant Methods 20, 62. DOI: https://doi.org/10.1186/s13007-024-01198-z

Lange, D. A., Penuela, S., Denny, R. L., Mudge, J., Concibido, V. C., Orf, J. H., & Young, N. D. (1998) A Plant DNA Isolation Protocol Suitable for Polymerase Chain Reaction Based Marker-Assisted Breeding. Crop Science, 38(1): 217. DOI: https://doi.org/10.2135/cropsci1998.0011183X003800010036x

Manoj, K., Tushar, B., & Sushama, C. (2007) Isolation and purification of genomic DNA from Black Plum (Eugenia jambolana Lam.) for analytical applications." International Journal of Biotechnology & Biochemistry, vol. 3, no. 1, Jan. 2007, pp. 49+. https://link.gale.com/apps/doc/A172131886/AONE?u=anon~80bfad9f&sid=googleScholar&xid=91ac90ff

Mundotiya N, Choudhary M, Jaiswal S, Ahmad U. (2023) Review of the Efficiency of Ten Different Commercial Kits for Extracting DNA from Soil Mixed Biological Samples. J Forensic Sci Res. 7: 017-024. DOI: https://doi.org/10.29328/journal.jfsr.1001045

Nadeem, M. A., Nawaz, M. A., Shahid, M. Q., Doğan, Y., Comertpay, G., Yıldız, M., Hatipoğlu, R., Ahmad, F., Alsaleh, A., Labhane, N., Özkan, H., Chung, G., & Baloch, F. S. (2018) DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing, Biotechnology & Biotechnological Equipment, 32:2, 261-285, DOI: https://doi.org/10.1080/13102818.2017.1400401

Nawawi, D. S., Akiyama, T., Syafii, W. & Matsumoto, Y. (2017) Characteristic of β-O-4 structures in different reaction wood lignins of Eusideroxylon zwageri T. et B. and four other woody species" Holzforschung, 71(1): 11-20. DOI: https://doi.org/10.1515/hf-2016-0100

Siran, A.S., N. Juliaty & B. Arti. (2004). Proseding ekspose hasil-hasil penelitian Balai Litbang Kehutanan Kalimantan. Badan Penelitian dan Pengembangan Kehutanan. Balai Litbang Kehutanan Kalimantan : Samarinda.

Stefanova, P., Taseva, M., Georgieva, T., Gotcheva, V., & Angelov, A. (2013). A Modified CTAB Method for DNA Extraction from Soybean and Meat Products. Biotechnology & Biotechnological Equipment, 27(3), 3803–3810. DOI: https://doi.org/10.5504/BBEQ.2013.0026

Turaki, A. A., Ahmad, B., Magaji, U. F., Abdulrazak, U. K., Yusuf, B. A., & Hamza, A. B. (2017). Optimised cetyltrimethylammonium bromide (CTAB) DNA extraction method of plant leaf with high polysaccharide and polyphenolic compounds for downstream reliable molecular analyses. African Journal of Biotechnology 16(24), 1354-1365.

Wilfinger, W. W., Mackey, K. and Chomczynski, P. (1997). Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity: BioTechniques 22: 474-481.

Zhang, X., Wang, L., & Shou, L. (2013). Modified CTAB method for extracting genomic DNA from wheat leaf. Agricultural Science & Technology, 14(7), 946-949. Retrieved from https://www.proquest.com/scholarly-journals/modified-ctab-method-extracting-genomic-dna-wheat/docview/1437308977/se-2

Unduhan

Diterbitkan

2024-06-29

Cara Mengutip

Ridzqya, S. A. A. ., Polosoro, A., Enggarini, W., Kusumanegara, K., Helmanto, H., Magandhi, M., Satyawan, D., Hadiarto, T., & Yuniaty, A. . (2024). Perbandingan Metode Ekstraksi DNA pada Tanaman Ulin (Eusideroxylon zwageri). Buitenzorg: Journal of Tropical Science, 1(1), 25–32. https://doi.org/10.70158/buitenzorg.v1i1.7

Terbitan

Vol 1 No 1 (2024): Buitenzorg: Journal of Tropical Science

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Articles

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Hak Cipta (c) 2024 Buitenzorg: Journal of Tropical Science

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